Journal: American Journal of Translational Research

Article Title: Endothelial progenitor cells with stem cells enhance osteogenic efficacy

doi:

Figure Lengend Snippet: ALP activity in the Bio-Oss granules with MSC-derived osteogenic cells, EPCs and co-cultured cells for 24 h, 3 days, 7 days, and 14 days. Results are presented as mean ± SD. *: P ≤ 0.05. ×: No expression.

Article Snippet: To quantify the mineralization in the cultures, an ALP substrate kit (Wako, Saitama, Japan) was used for the Bio-Oss granules with cells for 24 h, 3 days, 7 days, and 14 days according to the manufacturer’s instruction.

Techniques: Activity Assay, Derivative Assay, Cell Culture, Expressing

Journal: Calcified Tissue International

Article Title: Disruption of the Aldehyde Dehydrogenase 2 Gene Results in No Increase in Trabecular Bone Mass Due to Skeletal Loading in Association with Impaired Cell Cycle Regulation Through p21 Expression in the Bone Marrow Cells of Mice

doi: 10.1007/s00223-017-0285-0

Figure Lengend Snippet: Osteogenic potentials evaluated by cell culture assay using bone marrow cells flushed from the bilateral femurs and tibias on day 7: a number of total CFU-f colonies per well, b number of ALP + CFU-f colonies, and c percentage of ALP + /total CFU-f colonies. The data are shown as the mean ± SE ( n = 5 in each group). * p < 0.05

Article Snippet: On the 10th experimental day, CFU-f colonies were fixed with fixation solution and treated with a substrate tablet for ALP (BCIP-NBT solution kit for ALP stain, nuclease tested; Nacalai Tesque, Kyoto, Japan) dissolved in ddw at 37 °C.

Techniques: Cell Culture